Composite

Part:BBa_K136064:Design

Designed by: Benoît D   Group: iGEM08_Paris   (2008-10-28)


fliL promoter + WT RBS ( K136009) - EnvZ*


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 476
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 476
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 476
    Illegal BglII site found at 396
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 476
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 476
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Assembled biobricks


Source

the two parts of this construction were obtained by cloning from the genome of MG1655 WT and mutated for EnvZ bacteria strain

References